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| Registros recuperados: 42 | |
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| Pérez Ruíz, Elizabeth. |
| El Factor 9 de Crecimiento y Diferenciación (GDF9) es miembro de la súperfamilia β de factores de crecimiento (TGFβ) y su expresión en el ovocito es esencial para el desarrollo y crecimiento folicular. Diferentes mutaciones en el gen GDF9 han sido asociadas con el incremento de tasa de ovulación y/o prolificidad en algunas razas de ovejas, por lo que el objetivo de este estudio fue la búsqueda de polimorfismos de una sola base (SNPs) en el gen GDF9, así como la asociación entre polimorfismos del gen GDF9 y el índice de prolificidad en ovejas de la raza Pelibuey. Se tomaron muestras sanguíneas de la vena yugular de 16 ovejas y fueron conservadas en papel FTA® (Whatman Mini Card). El exón dos del gen fue amplificado mediante la técnica de Polimerase Chain... |
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Palavras-chave: Análisis de secuencias; GDF9; Mutación; Polimorfismo; PCR; SNP; Sequence analysis; Mutation; Polymorphism; Maestría; Ganadería. |
| Ano: 2012 |
URL: http://hdl.handle.net/10521/1712 |
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| Hernández Rodríguez, Martha. |
| El maíz (Zea mays L.) de calidad proteínica (QPM) es nutricionalmente superior en comparación con el maíz normal, porque produce semillas con casi el doble de los aminoácidos (aa) lisina (Lys) y triptófano (Trp), esenciales para la síntesis endógena de la vitamina B niacina. Actualmente, la conversión de una línea de maíz normal a una QPM, se acelera mediante la selección asistida por marcadores moleculares (MAS) y métodos de detección química. En este estudio se reporta el desarrollo y validación de marcadores basados en polimorfismos de un solo nucleótido (SNPs) para los loci opaque-2 (o2) y Ask-2, involucrados en la genética de los QPM, así como la interacción de ambos loci para el contenido de 11 aa en el endospermo de dos poblaciones F2 de maíz,... |
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Palavras-chave: Zea mays; Opaque-2; Ask-2; MAS; QPM; SNP; SSCP; Genética; Doctorado. |
| Ano: 2013 |
URL: http://hdl.handle.net/10521/2319 |
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| Lee,Youngsub; Park,Sairom; Kim,Hun; Lee,Seung Kyu; Kim,Jin Woo; Lee,Hak Kyo; Jeong,Dong Kee; Lee,Sung Jin. |
| Background: The melanocortin-4 receptor gene (MC4R) plays an important role in regulating food intake and body weight in mammals. In the present study, we identified the MC4R gene in native Korean brown, brindle, and black cattle by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP), and investigated its association with economic traits. A total of 413 cattle from the three breeds were tested for backfat thickness, carcass weight, longissimus muscle area, and marbling score, and statistical data were analyzed using the SAS program. Results: The C allele had the highest frequency in brown and brindle cattle, and the G allele frequency was highest in black cattle. The C1069G SNP was significantly (p < 0.05) associated with only... |
| Tipo: Journal article |
Palavras-chave: Economic trait; Marbling scores; MC4R gene; PCR-RFLP; SNP. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500014 |
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| Zhan,Xiaoli; Jiang,Bijie; Ma,Xianghui; Fu,Changzhen; Zan,Linsen. |
| Background: We investigated the polymorphisms of the bovine chemokine receptor-like 1(CMKLR1) gene. The coding region of CMKLR1 was screened in Qinchuan cattle by PCR-RFLP technology. Results: In this study, we discovered two single nucleotide polymorphisms (SNPs) (264G > C and 762C > T) in the coding region of the CMKLR1 gene. Hence, we described the BmgT120l and Pdm1 PCR-RFLP methods for detecting the 64G > C and 762C > T mutations, respectively. PCR-RFLP and sequencing were used to analyze the two loci of CMKLR1 gene in 324 individuals, which were randomly selected from breeding populations. Furthermore, meat quality traits in another 80 Qinchuan individuals were analyzed by the comparison between the genotypes and their phenotypic data.... |
| Tipo: Journal article |
Palavras-chave: CMKLR1 gene; Meat quality traits; PCR-RFLP; Qinchuan cattle; SNP. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000100003 |
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| Fu,Changzhen; Wang,Hong; Li,Yaokun; Wang,Jiali; Cheng,Gong; Wang,Hongbao; Yang,Wucai; Zan,Linsen. |
| Background CDIPT (CDP-diacylglycerol-inositol 3-phosphatidyltransferase, EC 2.7.8.11) was found on the cytoplasmic side of the Golgi apparatus and the endoplasmic reticulum. It was an integral membrane protein performing the last step in the de novo biosynthesis of phosphatidylinositol (PtdIns). In recent years, PtdIns has been considered to play an essential role in energy metabolism, fatty acid metabolic pathway and intracellular signal transduction in eukaryotic cells. Results In this study, the results of real-time polymerase chain reaction (PCR) showed that the expression of CDIPT gene was remarkably different in diverse tissues. We also detected the polymorphism of bovine CDIPT gene and analyzed its association with body measurement and meat quality... |
| Tipo: Journal article |
Palavras-chave: Body measurement traits; Gene expression; Meat quality traits; PCR-RFLP; SNP. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000400004 |
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| Biase,Fernando Henrique; Meirelles,Flávio Vieira; Gunski,Ricardo; Vozzi,Pedro Alejandro; Bezerra,Luiz A.F.; Vila,Reginaldo A.; Rosa,Artur J.M.; Lôbo,Raysildo B.; Martelli,Lúcia. |
| We sampled 119 Nelore cattle (Bos indicus), 69 harboring B. indicus mtDNA plus 50 carrying Bos taurus mtDNA, to estimate the frequencies of putative mtDNA single nucleotide polymorphisms (SNPs) and investigate their association with Nelore weight and scrotal circumference estimated breeding values (EBVs). The PCR restriction fragment length polymorphism (PCR-RFLP) method was used to detect polymorphisms in the mitochondrial asparagine, cysteine, glycine, leucine and proline transporter RNA (tRNA) genes (tRNAasn, tRNAcys, tRNAgly, tRNAleu and tRNApro). The 50 cattle carrying B. taurus mtDNA were monomorphic for all the tRNA gene SNPs analyzed, suggesting that they are specific to mtDNA from B. indicus cattle. No tRNAcys or tRNAgly polymorphisms were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Mitochondria; MtDNA; SNP; Weight. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600005 |
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| Huan,Pin; Zhang,Xiaojun; Li,Fuhua; Zhang,Yang; Zhao,Cui; Xiang,Jianhai. |
| Zhikong scallop Chlamys farreri (Jones et Preston) is an economically important species in China. Understanding its immune system would be of great help in controlling diseases. In the present study, an important immunity-related gene, the Lipopolysaccharide and Beta-1,3-glucan Binding Protein (LGBP) gene, was located on C. farreri chromosomes by mapping several lgbp-containing BAC clones through fluorescence in situ hybridization (FISH). Through the localization of various BAC clones, it was shown that only one locus of this gene existed in the genome of C. farreri, and that this was located on the long arm of a pair of homologous chromosomes. Molecular markers, consisting of eight single nucleotide polymorphism (SNPs) markers and one insertion-deletion... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chlamys farreri; LGBP; Fluorescence in situ hybridization (FISH); Indel; SNP. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100008 |
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| Raut,Ashwin A.; Kumar,Anil; Kala,Sheo N.; Chhokar,Vinod; Rana,Neeraj; Beniwal,Vikas; Jaglan,Sundeep; Samuchiwal,Sachin K.; Singh,Jitender K.; Mishra,Anamika. |
| Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The DGAT1 gene is a strong functional candidate for determining milk fat content in cattle. In this work, we used PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) and DNA sequencing to examine polymorphism in the region spanning exon 7 to exon 9 of the DGAT1 gene in Murrah and Pandharpuri buffaloes. Three alleles (A, B and C) and four novel single-nucleotide polymorphisms were identified in the buffalo DGAT1 gene. The frequencies of the alleles differed between the two buffalo breeds, with allele C being present in Murrah but not in Pandharpuri buffalo. The allele variation detected in this work may... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Buffalo; DGAT1; PCR-SSCP; Polymorphism; SNP. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400011 |
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| Martínez,Rodrigo; Bejarano,Diego; Gómez,Yolanda; Dasoneville,Romain; Jiménez,Ariel; Even,Gael; Sölkner,Johann; Mészáros,Gabor. |
| Abstract Genotypic and phenotypic data of 1,562 animals were analyzed to find genomic regions that potentially influence the birth weight (BW), weaning weight at seven months of age (WW) and yearling weight (YW) of Colombian Brahman cattle, with genotyping conducted using Illumina Bead chip array with 74,669 SNPs. A Single Step Genomic BLUP (ssGBLP), approach was used to estimate the proportion of variance explained by each marker. Multiple regions scattered across the genome were found to influence weights at different ages, also dependent on the trait component (direct or maternal). The most interesting regions were connected to previously identified QTLs and genes, such as ADAMTSL3, CAPN2, CAPN2, FABP6, ZEB2 influencing growth and weight traits. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bos indicus; SNP; QTL; GWAS; Body weight. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300453 |
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| Cassoli,Clarissa Sanches da Silva; Jorge,Erika Cirstina; Patrício,Mateus; Alves,Helena Javiel; Almeida,Erik Amazonas de; Ledur,Mônica Corrêa; Coutinho,Luiz Lehmann. |
| Brazilian poultry industry has reached a high level of development in both meat and egg production as a result of constant technological modernization. Further improvements can be achieved through genomics, but before this can be accomplished, a better understanding of gene expression profiles and nucleotide polymorphisms is necessary. Since animal physiology is directly or indirectly controlled by the pituitary and hypothalamus, the aim of the present work was to identify and analyze genes expressed in these tissues in chicken lines with different growth potential. Two pituitary and hypothalamus cDNA libraries from 21 day broiler (TT) and layer (CC) chickens lines were constructed and allowed identification of 3,074 unique sequences and 77 single... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pituitary; Hypothalamus; EST; SNP; Chicken. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600008 |
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| Pereira,Ítalo S; Barreto,Fernanda Z; Balsalobre,Thiago WA; Sala,Fernando C; Costa,Cyro P; Carneiro,Monalisa S. |
| A pungência em frutos de pimenta do gênero Capsicum, devido à presença de capsaicinóides, é uma das características mais atrativas e muito valorizadas pela culinária mundial. Neste trabalho, objetivou-se validar e avaliar a eficiência de predição dos marcadores moleculares pun1¹, pun1³ e o SNP identificado pelo método tetra-primer ARMS-PCR para a determinação de pungência em acessos de Capsicum spp. Trinta e seis acessos do Banco de Germoplasma da Universidade Federal de São Carlos foram avaliados através de análises sensoriais (em frutos maduros) e moleculares para pungência (na fase de plântula através da extração do DNA em folhas). Os marcadores SNP ARMS-PCR, pun1¹ e pun1³ foram usados na avaliação molecular. Os resultados mostram a eficiência desses... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Capsicum spp.; SNP; Melhoramento genético; Seleção.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362015000200009 |
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| Sauvage, Christopher; Bierne, N; Lapegue, Sylvie; Boudry, Pierre. |
| DNA sequence polymorphism and codon usage bias were investigated in a set of 41 nuclear loci in the Pacific oyster Crassostrea gigas. Our results revealed a very high level of DNA polymorphism in oysters, in the order of magnitude of the highest levels reported in animals to date. A total of 290 single nucleotide polymorphisms (SNPs) were detected, 76 of which being localised in exons and 214 in non-coding regions. Average density of SNPs was estimated to be one SNP every 60 bp in coding regions and one every 40 bp in non-coding regions. Non-synonymous substitutions contributed substantially to the polymorphism observed in coding regions. The non-synonymous to silent diversity ratio was 0.16 on average, which is fairly higher to the ratio reported in other... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Codon bias; Genetic diversity; SNP. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3417.pdf |
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| Harney, Ewan; Lachambre, Sebastien; Roussel, Sabine; Huchette, Sylvain; Enez, Florian; Morvezen, Romain; Haffray, Pierrick; Boudry, Pierre. |
| Selective breeding strategies require pedigree information over generations, but many species produced in aquaculture are too small to be physically tagged at early stages. Consequently, maintaining a sufficient number of separate families is often needed but costly and logistically difficult. Alternatively, parentage assignment can be obtained using DNA markers. We developed a panel of single nucleotide polymorphism (SNP) markers for the European abalone Haliotis tuberculata using an existing transcriptomic resource. An initial set of 2,176,887 SNPs was filtered to select 500 for high throughput genotyping. Of these, 298 SNPs were amplified in at least 90% of our H. tuberculata samples, consisting of a mixed family cohort (945 offspring) generated by... |
| Tipo: Text |
Palavras-chave: Abalone; Parentage assignment; Pedigree; Selective breeding; Transcriptome; SNP. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00429/54061/55396.pdf |
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| Griot, Ronan; Allal, Francois; Brard‐fudulea, S; Morvezen, R; Haffray, P; Phocas, F; Vandeputte, Marc. |
| In the context of parentage assignment using genomic markers, key issues are genotyping errors and an absence of parent genotypes because of sampling, traceability or genotyping problems. Most likelihood‐based parentage assignment software programs require a priori estimates of genotyping errors and the proportion of missing parents to set up meaningful assignment decision rules. We present here the R package APIS, which can assign offspring to their parents without any prior information other than the offspring and parental genotypes, and a user‐defined, acceptable error rate among assigned offspring. Assignment decision rules use the distributions of average Mendelian transmission probabilities, which enable estimates of the proportion of offspring with... |
| Tipo: Text |
Palavras-chave: Microsatellites; Missing parents; Parentage assignment; Pedigree; SNP. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00587/69866/67767.pdf |
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| Registros recuperados: 42 | |
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